The relationship between meal composition and long-term diet choice.

Abstract

When animals are offered a choice of feeds that are nutritionally complementary, they are able to select a consistent combination of these feeds over long periods of time. Analysis of how such consistent diet choice is achieved, in terms of short-term feeding behavior, may further our knowledge of how animals regulate nutrient intake. Previous work, on meal pattern analysis and on nutrient synchronization, led us to hypothesize that animals may select a consistent diet within a meal. In three experiments cows were offered a choice between high- (H) and low- (L) protein feeds and short-term feeding behavior data were collected using computerized feeders. Feeding behavior was first analyzed in terms of visit characteristics. A greater average daily intake of H, relative to L, was more closely related to the ratio of H visits to L visits than to differences in the intake per visit to feeders supplying H or L. Individual meal criteria were estimated using a mixed-distribution model, and visits were clustered into meals. Cows typically had approximately six meals per day. The observed frequency distribution of meal composition, in terms of the proportion of visits to H feeders, was determined. Subsequently, the observed visits were randomly reclustered into bouts consisting of the same number of visits as were observed in meals, and the frequency distribution of random bout composition was calculated. If frequency distributions of meals and random bouts coincide, then this is evidence that cows do not regulate diet choice within a meal. Comparison of the frequency distributions of meals and random bouts provided no evidence that cows attempted to achieve their long-term average diet composition within a meal. We also investigated whether cows tried to achieve a consistent diet choice within a meal by adjusting their intake per visit, depending on the feed type visited and the proportion of visits to H feeders in a meal. There was no evidence that this occurred. In conclusion, our analyses have shown that cows did not attempt to select within a meal a consistent diet in terms of protein to energy ratio. Indeed, our data and the literature suggest that the timeframe over which the intake of energy and protein is regulated must be greater than a meal in a number of animal species.