Abstract
A relationship between Hb Bart's levels in cord blood and the number of alpha-globin genes has been established by screening cord blood samples from 1,075 newborn babies. Diagnosis was made by gene mapping of DNA samples with restriction endonuclease digestion. The presence of Hb Bart's was determined with a discontinuous microelectrophoresis on cellulose acetate at pH 8.34. The establishment of this relationship allows an early diagnosis of alpha-thalassemia by this simple microelectrophoretic procedure.
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