Abstract
Objective: The quality of the embryo is greatly influenced by the quality of the oocytes; oocyte-secreted factors (OSFs), which include GDF-9 and BMP-15, play an important role in folliculogenesis. This study was to determine the relationship between GDF-9 and BMP-15 serum within the follicular fluid in order to predict the quality of oocytes in women undergoing In vitro Fertilization (IVF).
 Methods: We collected 30 samples of blood serum and 30 samples of follicular fluid on the day of ovum pickup (OPU), and examined GDF-9 and BMP-15 using ELISA kits. Analysis by Pearson and a partial-correlation was conducted to analyze the correlation between the concentration of GDF-9 and BMP-15 in serum and follicular fluid with general physiological parameters, such as maturation and fertilization rates.
 Results: The mean age of the subjects was 35,0(26,0-39,0) years. There was no statistical correlation between GDF-9 serum and follicular fluid (p=0.245); but there was a correlation between BMP-15 serum and follicular fluid (p=0.001). Average level of GDF-9 in the follicular fluid was 163,0 pg/ml (48,0-537), and average level in the serum was 260.33 pg/ml±121,82; average levels of BMP-15 in the follicular fluid was 58.30pg/ml±31,54 and average levels of BMP-15 in the serum was 74.20 pg/ml (1,0–610).
 Conclusion: There were no correlations between levels of GDF-9 serum-FF and BMP-15-FF, and maturation rates and fertilization rates. There was a negative correlation between BMP-15 serum and maturation rates.
Highlights
In vitro Fertilization (IVF) is a procedure wherein fertilization of the egg cell by the sperm is accomplished outside the body in vitro as an alternative method in infertility cases where other methods were unsuccessful [1]
There were abnormally distributed data (Shapiro-wilk) test for normality, P
There was no significant correlation between GDF-9 serum and FF (P>0,05) but there was a significant correlation BMP15 serum and follicular fluid (P
Summary
IVF is a procedure wherein fertilization of the egg cell by the sperm is accomplished outside the body in vitro as an alternative method in infertility cases where other methods were unsuccessful [1]. According to the Society for Assisted Reproductive Technology, data show that 11% of couples of reproductive age experience infertility, and approximately 10% of such cases require IVF with an average gestation of 21.3% and a birth-rate of 33.1% [4, 5]. Since the birth of the first IVF baby on July 25, 1978, IVF has become the most often-used assisted reproductive technology (ART) program, but the IVF process is not easy for couples and yields low success rates [1,2,3,4,5]. The quality of the embryo is greatly influenced by the quality of the oocytes; oocytes that are of poor quality can be the cause of infertility in women and a major obstacle to success with the IVF program. Several factors have been used as parameters to assess oocyte quality; the morphology parameters have much in used to search for a marker of the oocyte quality that can provide better potential for the development of the embryo
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
