The relationship between circulating anti-mullarian hormone (AMH) and superovulatory response in buffaloes

Abstract

The current study aimed to investigate the effect of using the superovulatory drug ‘follitropin’ on the follicular population and number of harvested buffalo embryos. At first, all animals were exposed to AMH analysis, Estrus was synchronized either by progesterone releasing intravaginal device (PRID) for 7 days or by double doses of cloprostenol sodium 11 days interval. A total dose of 40 IU of Follitropin was injected intramuscular for each animal as superovulatory drug (5 IU am and 5 IU pm for 4 consecutive days). AI was done using double straw/time for 3 times with 12 hours interval. Seven days later (day 0=ovulation day), embryos were recovered non-surgically. The results showed that total number of follicles/ animal was significantly increased in the group synchronized by PRID (7.4 ± 0.53) than the group synchronized by cloprostenol (4.8 ± 0.18), Number of the recovered embryos was significantly higher in the group synchronized by PRID (3.2 ± 0.71) than the group synchronized by cloprostenol (2.2 ± 0.37). In conclusion, AMH analysis is a crucial issue in expecting the superovulatory response in buffaloes, injection of follitropin as a superovulatory drug during diestrus phase in buffaloes that previously synchronized by PRID leads to a higher yielded embryo