Abstract
Objective To explore the interaction of microRNA-125b (miR-125b) and its target gene ERBB2 in glioma TJ905 cells.Methods Bioinformatic analysis found that miR-125b and ERBB2 has each other paired regions.We synthesised miR-125b and the control sequence,and constructed containing ERBB2 3' UTR of wild-type and mutant luciferase genes,and then transfected into TJ905 cells.We used the double luciferase reporter gene to analyze the function of miR-125b regulation on the 3'UTR region of the target gene ERBB2.48 h after transfection,protein was extracted,and the ERBB2 protein expression levels were analyzed by Western blotting.Results The bioinformatics analysis showed that miR-125b and ERBB2 3'UTR region has possible binding sites.Compared with the control group,miR-125binhibitor transfection group can up-regulate the expression of ERBB2 mRNA level was 1.7-fold higher than that of control group,the detection of ERBB2 gene Western blotting was about 1.3-fold higher than that of control group,while group niRNA-mimics ERBB2 mRNA fell about (37.19 ± 3.76) ％,the detection of ERBB2 Western blotting gene decreased by (39.87 ± 7.26)％.miR-125b can decline ERBB2 mRNA and protein levels via experimental verification.Conclusion ERBB2 is the target gene of miR-125b in glioma TJ905 cells,and miR-125b can be a negative regulator to ERBB2 expression. Key words: Glioma; ERBB2; MicroRNA-125b
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