The Regulation of Exogenous Jasmonic Acid on UV-B Stress Tolerance in Wheat

Abstract

Enhanced ultraviolet-B radiation (UV-B, 280–320 nm) is recognized as one of the environmental stress factors that cannot be neglected. Jasmonic acid (JA) is an important signaling molecule in a plant’s defense against biotic and abiotic stresses. To determine the role of exogenous JA in the resistance of wheat to stress from UV-B radiation, wheat seedlings were exposed to 0.9 kJ m−2 h−1 UV-B radiation for 12 h after pretreatment with 1 and 2.5 mM JA, and the activity of antioxidant enzymes, the level of malondialdehyde (MDA), the content of UV-B absorbing compounds, photosynthetic pigments, and proline and chlorophyll fluorescence parameters were measured. The results of two-way ANOVA illustrated that the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), MDA level, anthocyanin and carotenoid (Car) content, and almost all chlorophyll fluorescence parameters were significantly affected by UV-B, JA, and UV-B × JA (P < 0.05) [the maximal efficiency of photosystem II photochemistry (Fv/Fm) was not affected significantly by UV-B radiation]. Duncan’s multiple-range tests demonstrated that UV-B stress induced a significant reduction in plant photosystem II (PSII) function and SOD activity and an increased level of membrane lipid peroxidation, indicative of the deleterious effect of UV-B radiation on wheat. JA pretreatment obviously mitigated the detrimental effect of UV-B on PSII function by increasing Fv/Fm, reaction centers’ excitation energy capture efficiency (Fv′/Fm′), effective photosystem II quantum yield (ΦPSII), and photosynthetic electron transport rate (ETR), and by decreasing nonphotochemical quenching (NPQ) of wheat seedlings. Moreover, the activity of SOD and the content of proline and anthocyanin were provoked by exogenous JA. However, the MDA level was increased and Car content was decreased by exogenous JA with or without the presence of supplementary UV-B, whereas the contents of chlorophyll and flavonoids and related phenolics were not affected by exogenous JA. Meanwhile, exogenous JA resulted in a decrease of CAT and POD activities under UV-B radiation stress. These results partly confirm the hypothesis that exogenous JA could counteract the negative effects of UV-B stress on wheat seedlings to some extent.