Abstract
Ovarian cancer (OC) is the most lethal gynecologic malignancy and the top ten cancer of females in Taiwan and all over the world. Oncogenic activation of the Wnt signaling pathway is common in cancers, but mutation of beta‐catenin in ovarian cancer is rare. In addition to genetic events, epigenetic modification of secreted frizzled‐related protein (SFRP) family has been shown to be important in regulating Wnt signaling. The SFRPs act as antagonists of the Wnt ligands and inhibit the Wnt/β‐catenin signaling pathway. In the past research indicated the curcumin rich in curcuma longa has the capacity for anti‐ inflammatory, anti‐ oxidant and anti‐cancer effects as well as involve in the epigenetic regulation. It is helpful for inhibiting the tumor suppressor gene methylated and re‐expression. Therefore, the aim of this study is going to investigate the effects of curcumin on sFRP5 DNA methylation modification in ovarian cancer cell (SKOV3). Cell was treated with DMSO, 10 μM 5‐aza‐2′‐deoxycytidine (DAC), 5 μM DAC, 20 μM curcumin and respectively combined with 5 μM DAC in 96 hours, extracting the RNA and proteins for further analyze. The results of present study show that curcumin combined with 5 μM DAC may inhibit cancer cell colony formation and migration, the total DNMT activity, DNMT3a protein expression, also regulate tumor suppressor gene SFRP5 expression through Wnt/β‐catenin signaling pathway. As a result, it can attenuate or inhibit the ovarian cancer development.This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.
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