Abstract
A factor has been isolated from the serum of rats, 24 h after partial hepatectomy, which displays a capacity to stimulate DNA synthesis in the livers of normal mice. The factor is a heat stable protein with a molecular weight of approx. 26 000 and is liver specific. 400-fold purification of the protein has been achieved using ionexchange cellulose and Sephadex G-100 chromatography. The factor appears in the blood of partially hepatectomized rats some 12 h after the operation and remains at a high level for a further 12 h. The time course of the rise in DNA synthesis in mouse liver stimulated by the factor approximately parallels that observed in partially hepatectomized mice. The stimulation is also dose and time dependent. The assay for the factor and some of its characteristics are described, and mention is made of a material with similar effects isolated from fetal calf serum.
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