Abstract
This study focused on miR-494 inhibiting the proliferation, migration and invasion of cervical cancer cells by regulating HCCR1. During the study, 30 pairs of primary cervical cancer tissues and adjacent tissues diagnosed by pathology were collected, and then placed in a cryopreservation tube, immediately placed in liquid nitrogen to freeze, and then moved to a -80 °C refrigerator for storage until use. They were dipped with crystal violet staining solution for 20 minutes, washed 3 times with PBS, and dried at room temperature. The polycarbonate film on the chamber was gently cut, placed on a glass slide, and covered with neutral resin. The number of cells in 5 random fields was counted under a microscope, and the differences between the groups were compared. The qRT-PCR results showed that compared with the normal cervical tissue cell line HCCR1, the expression levels of miR-494 in cervical cancer cell lines HCCR1 and C-33A were significantly reduced. Compared with the transfection of mimics NC, the level of HCCR1mRNA and protein decreased significantly when transfected with miRNA-494mimics (P <0.05); compared with the transfection of inhibitor NC, the level of HCCR1 mRNA and protein increased significantly when transfected with miRNA-494 inhibitor (P <0.05).
Highlights
The specific mechanism of the disease is currently effective antioxidant, anti-inflammatory and antiunknown
This study focused on miR-494 inhibiting the proliferation, migration and invasion of cervical cancer cells by regulating HCCR1
Salidroside treatment caused changes in paper screens out the specific miRNA targeting the morphology of SiHa cells and induced cell cycle cervical cancer HCCR1, which provides a new arrest at the G2 / M and/or S phase, which was related theoretical basis for the targeted treatment of cervical to a significant decrease in the expression levels of cancer?
Summary
The specific mechanism of the disease is currently effective antioxidant, anti-inflammatory and antiunknown. Mohammad JahoorAlam et al / MiR-494 on HCCR1 in cervical cancer cells, 2021, 67(5): 131-137 concentrations of salidroside can induce apoptosis of SiHa cells, and its effect is dose-dependent. Several microRNAs have been described to regulate the process of angiogenesis [15,16] It shows that bone morphogenetic protein 4 (BMP4) increases the angiogenic activity of endothelial cells [17,18,19]. This article mainly studies the relationship between miR-494 and HCCR1, using RT-qPCR and Westernblot technology to prove the negative correlation between miRNA-494 and HCCR1 expression, and through bioinformatics analysis found miRNA-494 and HCCR1 Targeting relationship, and further verified by the dual-luciferase experiment On this basis, cell function experiments further confirmed that overexpression of miRNA-494 can target the expression of HCCR1, thereby inhibiting the proliferation, migration and invasion of cervical cancer cells
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