The refractive index and dry mass distribution of mammalian erythrocytes

Abstract

SUMMARYQuantitative microscopical methods are now of outstanding importance in the diagnosis and assessment of mammalian red blood cells.After release from the erythropoetic organs, all red blood cells in the peripheral blood of mammals pass through the reticulocyte stage before becoming mature erythrocytes. Therefore, the determination of reticulocyte number is used as an index of erythropoetic activity in clinical hæmatology.The corpuscular solid concentration, the corpuscular dry weight, the osmotic resistance and the size of red blood cells can be determined by use of microscopical methods. For the determination of the corpuscular solid concentration of erythrocytes, cell refractometry by the use of phase‐contrast microscopy has proved to be a very sensitive and accurate method. The dry weight of single erythrocytes may likewise be obtained by the use of interference microscopy with relatively high accuracy. Both of these methods might also be of use in clinical hæmatology.In the experimental work described in this paper, both methods mentioned above are used to answer the question: whether and under what conditions the refractive index as a criterion of measurement of the solid concentration and the relative dry weight can be determined separately for reticulocytes and erythrocytes from the same blood sample by use of microscopical methods alone. The frequency distributions of refractive indices and relative dry weights determined on human reticulocytes and mature erythrocytes show significantly lower mean values for the reticulocyte generation in accordant with findings published by other authors and obtained by use of sometimes entirely different methods. The additional information gained by the determination of the frequency distribution of refractive index and dry weight of reticulocytes and mature erythrocytes separately in a blood sample and the necessity for simplifying and automating such methods for hæmatological diagnosis are discussed.