Abstract
The use of reference strains is a critical element for the quality control of different assays, from the development of molecular methods to the evaluation of antimicrobial activities. Most of the strains used in these assays are not type strains and some of them are cited erroneously because of subsequent reclassifications and descriptions of novel species. In this study, we propose that the reference strain Aeromonas hydrophila CIP 57.50 be reclassified as Aeromonas salmonicida CIP 57.50 based on phenotypic characterization and sequence analyses of the cpn60, dnaJ, gyrB and rpoD genes.
Highlights
The use of reference strains is a critical element for the quality control of different assays, from the development of molecular methods to the evaluation of antimicrobial activities
We propose that the reference strain Aeromonas hydrophila CIP 57.50 be reclassified as Aeromonas salmonicida CIP 57.50 based on phenotypic characterization and sequence analyses of the cpn60, dnaJ, gyrB and rpoD genes
The most recent taxonomic opinion places this strain as a member of the genus Eggerthella (Wade et al, 1999). This problem can occur at an interspecific level if the strain used for quality control is not the type strain, for example, Aeromonas hydrophila ATCC 7965 (5NCTC 78125CIP 57.50) is a reference strain frequently used to assess antimicrobial susceptibilities, the sensitivity and specificity of PCR protocols and stress responses, among other assays (Isonhood et al, 2002; Huddleston et al, 2006; Skocibusicet al., 2006; Al Amri et al, 2007; Giddens & Bean, 2007; Gordon et al, 2008)
Summary
Departament de Microbiologia i Parasitologia Sanitaries, Facultat de Farmacia, Universitat de Barcelona, Av. The most recent taxonomic opinion places this strain as a member of the genus Eggerthella (Wade et al, 1999) This problem can occur at an interspecific level if the strain used for quality control is not the type strain, for example, Aeromonas hydrophila ATCC 7965 (5NCTC 78125CIP 57.50) is a reference strain frequently used to assess antimicrobial susceptibilities, the sensitivity and specificity of PCR protocols and stress responses, among other assays (Isonhood et al, 2002; Huddleston et al, 2006; Skocibusicet al., 2006; Al Amri et al, 2007; Giddens & Bean, 2007; Gordon et al, 2008). We have performed an extensive phenotypic characterization, PCR amplification and sequencing of the 16S rRNA, cpn, dnaJ, gyrB and rpoD genes for A. hydrophila strain CIP 57.50 (5ATCC 79655NCTC 7812) using previously described methods (Soler et al, 2004; Minana-Galbis et al, 2007, 2009; Nhung et al, 2007). Sequence alignments and distances (Jukes–Cantor model and pairwise deletion option) were calculated with MEGA4 software
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