The reduction of the Rieske iron–sulfur cluster in naphthalene dioxygenase by X-rays

Abstract

Naphthalene 1,2 dioxygenase (NDO) displays characteristic UV–Vis spectra depending on the oxidation state of the Rieske center. Investigations on crystals of NDO grown for X-ray diffraction experiments showed spectra characteristic of the oxidized form. Crystals reduced in an anaerobic glovebox using sodium-dithionite showed a characteristic reduced spectrum. Spectra of crystals (cooled to 100 K) after being exposed to X-rays for data collection showed spectra corresponding to a reduced Rieske iron center, demonstrating the ability of X-rays to change the oxidation state of the Rieske iron–sulfur cluster in NDO.