Abstract
It has previously been shown that stimulation of class I MHC antigen expression by human interferon gamma (Hu-IFNgg) depends on the presence of both human chromosomes 6 and 21. Even although the presence of human chromosome 6 results in normal expression of the IFNγ receptor on the cell surface, chromosome 21 is required for this biological response. Here, we transfected the cDNA encoding the Hu-IFNγ receptor into a mouse cell line, L(tk −), and into a mouse × human hybrid, GMO8, containing the human chromosome 21 as the only human genetic material. Two clones, L-γR.8 and GMO8-γR.9, were tested for different biological responses to Hu-IFNγ. Only the hybrid containing the human chromosome 21 (GMO8-γR.9) exhibited a biological response when assayed for the stimulation of murine class I MHC antigen expression by Hu-IFNγ. Moreover, transcriptional induction of 2′-5′-oligoadenylate synthetase and 1–8 genes, as well as antiviral protection, were observed only in the GMO8-γR.9 transfectants. These results show that human chromosome 21 encodes a factor which mediates different biological activaties of Hu-IFNγ.
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