Abstract
In view of its probable wide applicability, it seems desirable to publish a note on a simple technic for the recognition with the microscope of the action of lipase. In brief, the method is to make an emulsion of neutral fat previously stained with a red Sudan stain, subject some of the emulsion to the action of the supposed lipase for an appropriate time and then examine with the microscope a recovered drop of the emulsion in a solution of Nile blue sulfate. It has long been known that Nile blue sulfate stains liquid neutral fats a reddish color and fatty acids blue.
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