THE REACTION OF PERIPHERAL BLOOD OF EXPERIMENTAL ANIMALS TO COMBINED EFFECTS OF LOW-INTENSITY LASER END γ-RADIATION

Abstract

By the response of the peripheral blood of experimental Wistar rats to the combined action of γ and laser radiation, the radioprotective effect of laser radiation was studied. Four series of experiments were carried out, in each of which the whole body of rats was irradiated once with γ-radiation (dose 3 Gy). Low-intensity laser exposure was performed in the form of blood overvein irradiation (NLOK), which differed in the different series of experiments by the number of procedures, the laser radiation density (6.25 J/cm 2 , 2.5 J/cm2, 1.25 J/cm 2 ) and the exposure sequence: before and after γ-irradiation. The concentration of hemoglobin, hematocrit, the number of erythrocytes, leukocytes, lymphocytes, platelets were determined. Along with the study of the effect of laser radiation on the radiation changes in hematological parameters, changes were also analyzed: the activity of antioxidant defense enzymes (superoxide dismutase and catalase). It is shown that laser radiation tends to compensate the effect of γ-radiation, which caused leukopenia and lymphopenia. Significant differences in the individual radio sensitivity of blood samples of individual rats were revealed. For blood constituents and antioxidant defense enzymes, it was demonstrated the dependence of the quantitative changes caused by laser and γ-radiation on the initial indices.