Abstract
Mustard gas was allowed to react with DNA at pH 7.4. After mild acid hydrolysis of the product, the mustard (estimated as sulphur) was found in three fractions: bound to purine, bound to apurinic acid, and a portion bound to neither and whose origin was not apparent. The apurinic acid was degraded to free pyrimidines by perchloric acid hydrolysis. From the hydrolyzate, a cytosine and a thymine derivative were isolated and characterized spectrophotometrically. Protamine titration of the DNA–mustard gas product indicated that primary phosphoryl groups had been esterified. It is difficult to reconcile this conclusion with a previous finding that phosphate esterification did not occur. The latter result was obtained by measuring the release of hydrogen ion during reaction.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
