The reaction of influenza viruses with guinea pig polymorphonuclear leucocytes. II. The reduction of white blood cell glycolysis by influenza viruses and receptor-destroying enzyme (RDE)

Abstract

Influenza viruses, PR8 and Lee, markedly inhibited anaerobic glycolysis when glucose served as substrate. Inhibition of CO 2 evolution corresponded to a reduction in lactic acid produced. Reduction of glycolysis was accomplished by the viral particles and was proportional to the viral concentration employed. Whereas receptor-destroying enzyme of Vibrio cholera (RDE) also inhibited glycolysis of white blood cells, neither heat-inactivated RDE nor virus inhibited this metabolic process. Calcium was not essential for adsorption of virus to white blood cells, but this cation was required for inhibition of glycolysis. Inhibition of glycolysis by influenza virus was obtained when glucose or glucose-6-phosphate was employed as substrate, but not when fructose-6-phosphate or fructose-1, 6-diphosphate was utilized. Periodate also inhibited glycolysis of white blood cells but the metabolic pattern was not similar to that obtained with influenza viruses or RDE.