Abstract
In the hexokinase reaction with equilibrated d‐glucose (36.2%, α‐d‐glucopyranose and 63.8%β‐d‐glucopyranose) at saturating concentrations of ATP‐Mg, the ratio of phosphorylation of α‐d‐glucose and β‐d‐glucose was investigated.It is shown that, knowing the Michaelis constants Km,(α), Km,(β), and the maximum velocities V(α), V(β), of hexokinase with α‐d‐glucose and β‐d‐glucose respectively, one can calculate the ratio of phosphorylation of α‐d‐glucose and β‐d‐glucose starting from the equilibrium mixture of d‐glucose.In addition, the ratio of generation of α‐and β‐d‐glucopyranose‐6‐phosphate in the hexokinase reaction was determined from steady‐state analysis of the hexokinase/glucose‐6‐phosphate dehydrogenase system with equilibrated d‐glucose. The experimentally obtained and the calculated values were in agreement.
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