THE RATIO OF TrkA AND p75 RECEPTORS ON THE CELL NEUROEPITHELIAL TUMORS – A NEW TARGET EFFECT OF NERVE GROWTH FACTOR WITH CISPLATIN AND TEMOZOLOMIDE AND THE COMBINATIONS

Abstract

Objective. To study the change of expression of TrkA, p75 receptors and their ratio on the human anaplastic astrocytoma, glioblastoma and medulloblastoma cells cultures exposed to nerve growth factor (NGF) and its combination with cisplatin or temozolomide, to establish participation of expression of the receptors in sensitivity tumor cells to NGF and its combinations with chemotherapeutical drugs. Materials and methods . In primary cell cultures of anaplastic astrocytoma, glioblastoma and medulloblastoma were studied: 1) cytotoxicity effects of nerve growth factor and its combination with cisplatin or temozolomide; 2) the expression of TrkA, p75 receptors and the its ratio by immunofluorescence; 3) correlation between expression of TrkA, p75 receptors, the its ratio and index of cytotoxicity of reagents. Results. It is found that the expression of the receptors and the relationship depends on the tumor type and reagents’ mechanism of action. Expression of TrkA and p75 receptors and their ratio correlated with sensitivity of anaplastic astrocytoma and glioblastoma cells to NGF and its combination with cisplatin or temozolomide. Conclusion. Relative expression of TrkA/p75 receptor, and its coefficient of correlation with index of cytotoxicity point to a mechanism (signaling pathway), on which there is cell death.