The ratio of expression of angiotensin receptors type 1 (AT1), type 2 (AT2) mRNA in local endometriosis lesions and eutopic endometrium

Abstract

48h. Immortalizedfirst trimester human trophoblastHTR8 cells were plated on matrigel chambers with various concentrations ofP. gingivalis sup.NK-92MI cellswere cultured withP. gingivalis supandcytotoxicityagainstK562cells and TNFproductionwere estimatedwithflowcytometry and ELISA respectively. Results: Matrigel invasion of HTR8 was inhibited by P. gingivalis sup in a concentration dependent manner. The inhibition was observed at non-cytotoxic concentrations. P. gingivalis sup also induced de novo production of TNFbymucosal CD56+ NK cells but was not sufficient to induce natural killer activities. Conclusions: Culture supernatant of P. gingivalis contains inhibitory substance(s) on early trophoblast invasion into myometrium and inflammatory cytokine production of mucosal natural killer cells. Our observations suggest very early events in human pregnancy are susceptible for periodontitis through direct functional suppression of trophoblastic invasion and inflammatory cytokine production by NK cells. In this sense, dental care for periodontitis must be done before pregnancy to reduce pregnancy complications.