Abstract
The rates of dissociation and recombination of the subunits of human luteinizing hormone have been measured under a variety of conditions by exploiting the fact that the fluorescence probe 1,8-anilinonaphthalene sulfonate binds to the native hormone but not to its subunits. The first-order dissociation rate is strongly dependent on temperature, pH, and urea concentration. Similarly, the rate of recombination depends on pH and temperature and is independent of subunit concentration in the range from 10 to 40 μ m. At 37 °C, the subunits dissociate below pH 4 and recombine above pH 5. The results presented herein should be useful for developing improved methods of purifying human luteinizing hormone and its subunits.
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