Abstract
The rate of deoxygenation of red blood cells has interested many investigators [2-151 since Roughton opened the field in 1932 [l] . It has been shown that the same factors which affect the dissociation of oxygen from hemoglobin in solutions, such as temperature, pH and carbon dioxide, exert similar effects in intact red blood cells [8,9]. The recent elucidation of the influence of organic phosphates on the equilibrium between oxygen and hemoglobin, both in solution [ 16, 171 and in red blood cells [ 181, as well as their effects on the kinetics of oxygen association and dissociation of hemoglobin solutions [ 19,201, led us to explore the effect of 2,3diphosphoglycerate (2,3-DPG) on the rate of deoxygenation of intact human red blood cells. As a highly charged anion at physiologic pH, 2,3-DPG affects the distribution of protons across the cell membrane as predicted by Gibbs-Donnan theory [21]. Therefore, alterations in the content of 2,3-DPG will significantly modify intracellular PH. In order to isolate the effects of
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