Abstract
A method is described that allows the rapid determination of equilibrium binding constants for antibody-antigen reactions. The method, based upon the radioimmunoassay procedure of Rosenthal et al. (1973), has been applied to the interaction of monoclonal antiviral hybridoma antibodies with a panel of influenza viruses. Equilibrium binding constants with values from 10 6 M −1 to 10 10 M −1 have been observed with errors of approximately ±6.5%. An independent determination by equilibrium dialysis of the binding constant of one hybridoma antibody is in excellent agreement with the results presented here. Heterogeneity constants for the reaction pairs are also presented and are consistent with the interaction of monoclonal antibodies with homogeneous protein determinants.
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