Abstract
A rapid procedure for the extraction and assay of γ-aminobutyric acid (GABA) is described. The extraction procedure prevents rapid GABA accumulation during the sampling of tissue for analysis. It also removes over 95% of pigments absorbing at 340 nm which otherwise reduce the sensitivity of the spectrophotometric coupled enzyme assay. The absorbance increase at 340 nm was linear for GABA levels ranging from 10 to 100 nmol per cuvette.
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