Abstract
Hydrogen sulfide (H2S) is a gaseous signaling molecule that participates in various physiological processes such as regulation of blood pressure. Cystathionine beta‐synthase (CBS) and gamma‐cystathioninase (CSE) are the two enzymes that are responsible for the formation of H2S in the transsulfuration pathway. Quantitative Western blot analysis was used to determine the relative abundance of each enzyme in murine liver and kidney. In this study, we report quantification of CBS and CSE in murine liver and kidney and their contribution to H2S generation in these tissues and in brain. Our results show that the level of CBS protein is significantly lower than CSE; 60‐fold and 20‐fold in liver and kidney respectively. Levels of both enzymes are higher in liver compared to kidney, 2‐fold and 6‐fold for CBS and CSE respectively. At high substrate concentrations (20 mM each cysteine and homocysteine), CBS and CSE contribute equally to H2S production in liver extract while CBS constitutes the major source of H2S in kidney and brain with CSE contributing approximately 20% and 6% respectively. Determination of tissue levels of these enzymes is important for therapeutic reasons that aim to specifically modulate H2S synthesis in tissue‐specific manner.
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