Abstract
Oxindole alkaloids occurring in various species of Mitragyna were determined quantitatively by means of ultra-violet spectrophotometry, colorimety and densitometry after extracting the total alkaloid from the plant material and separating the individual alkaloids by thin-layer chromatography. The results showed that if there were few alkaloids in the plant there was little to choose between the three methods and that in each case the coefficient of variation was small but if a large number of alkaloids were present the results from all three methods showed greater coefficients of variation, those being obtained by densitometry being less reliable than the others.
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