Abstract

Techniques are described for the quantitative analysis of biological solutions for galactose content, involving incubation of unknown samples with the enzyme galactose oxidase in the presence of peroxidase and benzidine. Some characteristics of the reaction are discussed and conditions are defined for two procedural variations, depending upon the sensitivity and range desired. Directions are stated for the application of the method to blood, urine, and other biological fluids, and illustrations of a number of these applications are presented.

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