Abstract
We compared Quebracho with Sorghum tannin as standards for condensed tannin (CT) quantification in selected African savanna tree species in relation to the acid-butanol assay for CTs. Without exception, the use of Quebracho tannin as standard overestimated CTs, ranging from 0.7 to as much as 8.3 times. Sorghum tannin underestimated CTs by 0.26–0.79 times, except in one species where there was no difference in the CT concentration. Condensed tannins in African savanna trees showed qualitative and quantitative differences in chemical composition which may explain the variable reactivity in the acid-butanol assay. We propose the use of condensed tannins purified from the plant under investigation be used as standard since it will closely represent the CT structure and presumably chemical reactivity in the acid-butanol assay.
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