Abstract

The lactate dehydrogenase ( l-lactate:NAD oxidoreductase, EC 1.1. 1.27; LDH) isoenzyme content of 31 commercially available quality control sera was determined using thin layer agarose and fluorimetry. Only one of these sera was specifically intended for isoenzyme quality control purposes. The group of human sera, with no additives, proved to be the only one having an LDH isoenzyme distribution suitable for quality control use. Within-batch and between-batch precision has been determined over a 2–3 month period for LDH isoenzyme separations using several human quality control sera. The control of quality of this procedure has been shown to be feasible.

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