Abstract

The selectivity of chromatographic separation of tobacco mosaic virus nucleoproteins on ECTEOLA-Cellulose has been tested with an electrophoretically heterogeneous virus preparation. The fractions of a single run did not differ in their ultraviolet spectra and in their infectivities. The heterogeneity was only revealed after the chromatographic purification was repeated with the head fractions. By comparison of the elution diagrams it could be demonstrated that after three chromatographic runs about 10% of contaminants had been removed. The two very similar main nucleoprotein components shown in the electrophoresis, however, were not separated.

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