Abstract
Pregnancy-specific β 1 2-glycoprotein was purified to 99% purity by sequential chromatography on carboxymethyl-cellulose (to remove other pregnancy-specific β 1-glycoprotein variants), Cibacron blue 3G-A-Matrex Gel, Procion turquoise MX-G-Matrex Gel, Procion red H-8BN-Matrex Gel, and rabbit anti-normal human male serum-Matrex Gel. The purification was rapid, large-scale, and gave a yield of pregnancy-specific β 1-glycoprotein of 35.1%.
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