Abstract
Concanavalin A was demonstrated to react with 3 antigenic polysaccharide constituents of culture filtrates of Mycobacterium tuberculosis. Using direct precipitation and affinity chromatography methods, concanavalin A-reactive polysaccharides could be separated from concanavalin A-non-reactive polysaccharide in a partially purified, polysaccharide-rich fraction of mycobacterial culture filtrate. By the use of appropriate dissociation conditions, 2 concanavalin A-reactive polysaccharides of differing concanavalin affinities could be separated and purified. Four polysaccharides thus studied were identified with the United States-Japan mycobacterial antigen nomenclature. Antigen 1 was found to be a concanavalin A-reactive polysaccharide of high affinity. Antigen 2 was found to identify both with a concanavalin A-reactive polysaccharide of low affinity and with a concanavalin A-nonreactive polysaccharide. Antigen 3 was found to represent the large molecular weight polysaccharide II of Seibert and to react with...
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