Abstract
Maize dwarf mosaic virus (MDMV) was diagnosed on corn crop in Ninewah province by using tissue blot immunoassay (TBIA). The virus isolate was propagated in corn plants. The virus was purified by differential centrifugation and precipitated by polyethylene glycol (PEG) and then by sucrose density gradient. The efficiency of purification method was evaluated biologically and spectrometrically, and virus purity was 1.12 . Its antiserum was prepared by using rabbit, the end the dilution point ( titer ) of antiserum was 1/ 512. The efficiency of antiserum was tested by agglutination, and agar double diffusion tests .
Highlights
الملخص تم تشخيص فايروس موزائيك وتقزم الذرة ) Maize dwarf mosaic virus (MDMVعلـى الـذرة الصفراء في حقول من محافظة نينوى باختبـار بـصمة النـسيج المناعيـة Tissue Blot Immunoassay ) .(TBIAوتم إكثار عزلة الفايروس على نباتات الذرة الصفراء .تمت تنقية الفـايروس بالانتبـاذ التنـاوبي والترسيب بمادة الجلايكول متعدد الايثيلين )
The virus isolate was propagated in corn plants. The virus was purified by differential centrifugation and precipitated by polyethylene glycol (PEG) and by sucrose density gradient
Its antiserum was prepared by using rabbit, the end the dilution point of antiserum was 1/ 512
Summary
الملخص تم تشخيص فايروس موزائيك وتقزم الذرة ) Maize dwarf mosaic virus (MDMVعلـى الـذرة الصفراء في حقول من محافظة نينوى باختبـار بـصمة النـسيج المناعيـة Tissue Blot Immunoassay ) .(TBIAوتم إكثار عزلة الفايروس على نباتات الذرة الصفراء .تمت تنقية الفـايروس بالانتبـاذ التنـاوبي والترسيب بمادة الجلايكول متعدد الايثيلين )
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