Abstract
Papain, a proteolytic enzyme was purified from latex of Carica papaya by a variety of techniques such as two-step salt precipitation, organic solvent precipitation (ethanol) and ion exchange chromatography. Fold purity obtained using ion exchange chromatography was in the range of 4.5 to 5 times as compared to the precipitation techniques. The purified papain was entrapped in ionotropically cross-linked alginate beads, in order to develop formulation for site-specific delivery of papain in intestine. The entrapment efficiency for papain in alginate beads was found to be in excess of 86%. Drying at low temperature and low air velocity results into maximum retention of papain activity; however, drying time increases significantly. The dissolution study shows the possible use of alginate beads for site-specific intestinal delivery of papain. The shelf-life of papain can be improved significantly by entrapment in alginate matrix, which was confirmed by an FTIR study.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.