Abstract
The tumor suppressor PTEN is disrupted in a large proportion of cancers, including in HER2-positive breast cancer, where its loss is associated with resistance to therapy. Upon genotoxic stress, ataxia telangiectasia mutated (ATM) is activated and phosphorylates PTEN on residue 398. To elucidate the physiological role of this molecular event, we generated and analyzed knock-in mice expressing a mutant form of PTEN that cannot be phosphorylated by ATM (PTEN-398A). This mutation accelerated tumorigenesis in a model of HER2-positive breast cancer. Mammary tumors in bi-transgenic mice carrying MMTV-neu and Pten398A were characterized by DNA damage accumulation but reduced apoptosis. Mechanistically, phosphorylation of PTEN at position 398 is essential for the proper activation of the S phase checkpoint controlled by the PI3K–p27Kip1–CDK2 axis. Moreover, we linked these defects to the impaired ability of the PTEN-398A protein to relocalize to the plasma membrane in response to genotoxic stress. Altogether, our results uncover a novel role for ATM-dependent PTEN phosphorylation in the control of genomic stability, cell cycle progression, and tumorigenesis.
Highlights
PTEN contains an N-terminal phosphatase domain that can dephosphorylate a component of the lipid cellular membrane, phosphatidylinositol 3,4,5-trisphosphate (PI(3,4,5)P3 or PIP3) [1]
We discovered that ataxia telangiectasia mutated (ATM) phosphorylates PTEN at position 398 upon activation of the DNA damage response (DDR) [27]
To assess whether the impaired ability to resolve DNA damage foci in cells expressing PTEN-398A is due to defects in specific DNA repair pathways, we evaluated the efficiency of Homologous recombination (HR) and non-homologous end-joining (NHEJ), using reporter assays
Summary
PTEN contains an N-terminal phosphatase domain that can dephosphorylate a component of the lipid cellular membrane, phosphatidylinositol 3,4,5-trisphosphate (PI(3,4,5)P3 or PIP3) [1]. By dephosphorylating the D3 position of PIP3, Edited by G.
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