Abstract
Pseudo-curly top virus (PCTV) was transmitted by infective treehoppers ( Micrutalis malleifera Fowler) to the following species of plants: ragweed ( Ambrosia sp.), chickweed (Stellaria medea L.), tomato (Lycopersicum esculentum Mill), nightshade ( Solanum nigrum L.), tobacco ( Nicotiana glutinosa L.) , and jimsonweed ( Datura stramonium L.). Symptoms in these plants were similar to those caused by other curly top viruses. Vector-virus relationships were studied. The acquisition threshold and inoculation threshold periods were both less than 1 hour. A latent period of between 24 and 48 hours was demonstrable. Virus retention was correlated with length of access feeding. Treehoppers given 24-hour access feeding periods acquired a higher charge of virus and also remained infective longer than those given 1 hour access feedings. There was no indication of virus multiplication in the vector. The half life of the virus was calculated to be 33 days. The vector-virus relationships resembled those of the beet leafhopper, Circulifer tenellus (Baker), and western curly top virus. No differences in vector efficiency were found between sexes.
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