Abstract
The Proteins of Bovine Pancreatic Juice
Highlights
By means of ion exchange chromatography, approximately 90 per cent of the protein of bovine pancreatic juice can be recovered as discrete components, and about 76 per cent of the protein can be related to known pancreatic proteins
A large proportion of the total proteins of bovine pancreatic juice is composed of proteolytic enzymes or their respect.ive zymogens
A chromatographic system has been develcped for the quantitative separation of the protein components of bovine pancreatic juice, obtained from steers with a permanent pancreatic fistula
Summary
The choice of bovine pancreatic juice as starting material was based on two considerations: (a) all of the proteins cited are components of the extracellular secretion of the pancreas gland; [5] since the crystalline pancreatic proteins have been prepared routinely from beef glands, the problem can be circumvented. The concentration of phosphate buffer in the effluent fractions containing the maximal concentration of eluted protein was measured, and found to be 0.05 M for Component 2 and 0.048 M for chymotrypsinogen B. Is not adsorbed at this pH on the anion exchange resin, DEAE-cellulose For study of their enzymatic properties, solutions of the respective chymotrypsinogens were prepared in 0.04 M phosphate buffers, pH 8.0. The sedimentation constants of components 7 and 8 were measured at a single protein concentration of approximately 1 per cent and found to correspond to the sedimentation constant of procarboxypeptidase-A (5.86 Svedberg units). Trypsinogen and chymotrypsinogen-a were poorly resolved at this pH, but a component corresponding chromatographically and in activity to ribonuclease-A was found to exist in the pancreatic juice. No component corresponding to ribonuclease-B was seen [28], it cannot be said in the light of these experiments that this protein is not present in
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