The protein P23 identifies capsule-forming plasmatocytes in the moth Pseudoplusia includens

Abstract

The moth Pseudoplusia includens produces four types of hemocytes named granulocytes, plasmatocytes, spherule cells and oenocytoids. Prior studies established that the main function of plasmatocytes in P. includens is encapsulation of parasitoids and other foreign entitites. P. includens plasmatocytes are also recognized by several monoclonal antibodies that bind unknown antigens. Of particular interest is the antibody 43E9A10 whose binding properties indicate that plasmatocytes consist of two subpopulations: cells that can spread on foreign surfaces and cells that cannot. Here we report 43E9A10 recognizes P23, which is a member of the aegerolysin protein family. Expression analyses confirmed that p23 is specifically expressed in plasmatocytes. Functional studies indicated that only P23-expressing plasmatocytes form capsules and spread in response to the cytokine plasmatocyte spreading peptide. In contrast, P23 showed no antibacterial or cytolytic activity toward bacteria and mammalian erythrocytes. Overall, our results suggest that P23 is a maturation marker that identifies capsule-forming plasmatocytes.