The protein expression of Survivin, b-FGF and Ang-1 in tissues of colorectal cancer and relation with tumor angiogenesis

Abstract

Objective To investigate the expression of Survivin,basic fibroblast growth factor and angiopoietin-1 in colorectal cancer and delineate the relationship of expression levels of protein with tumor angiogenesis.Methods The expression of Survivin,b-FGF,Ang-1 and CD34 were detected in 7 cases of colitis tissues,10 cases of intestinal polyps and 26 cases of colorectal cancer tissues by immunohistochemical methods.Results In colorectal cancer group,the positive expression rates of Survivin and Ang-1 protein were 76.9％ and 80.8％,respectively,which were significantly higher than that in both colorectal polyps group and colitis group (P＜0.05).In colorectal cancer group,the positive expression rate of b-FGF protein was 73.1％,which was significantly higher than that in colitis group (P ＜ 0.05),but which was not statistical significance in comparison with colorectal polyps group (P ＞ 0.05).In colorectal cancer group,CD34 protein microvessel count was 23.69±9.96,which was significantly higher than that in both colorectal polyps group and colitis group.In colorectal cancer group,the expression of Survivin,b-FGF,Ang-1 protein showed positive correlation with tumor MVD.The expression of Survivin and b-FGF,Ang-1 in colorectal cancer tissues related closely.Conclusion In colorectal cancer development and advancement,Survivin,b-FGF and Ang-1 paly important roles with neovascularization. Key words: Survivin; b-FGF; Ang-1; CD34; Colorectal cancer; Immunohistochemistry