Abstract
Objectives. In the present study, we investigated the protective effects of Shen-Fu injection (SFI) on a caerulein-induced rat pancreatitis (AP) model. Methods. SFI was given to rats in the SFI treated group through intraperitoneal injection. Blood and pancreas samples were collected for serological and histopathological studies. Results. Our results showed that AP caused significant decrease in tissue glutathione (GSH) and serum IL-4 and IL-10, while pancreatic malondialdehyde (MDA) and myeloperoxidase (MPO) were increased. Furthermore, TNF-α, IL-1β, amylase, and lipase levels were also significantly increased. On the other hand, SFI treatment reserved all these biochemical indices as well as histopathologic alterations that were induced by caerulein. Conclusion. Our findings suggest that the SFI protects against caerulein-induced AP in rats via modulation of cytokines, oxidative stress, and Nuclear Factor-kappa B (NF-κB) activity.
Highlights
Acute pancreatitis (AP) involves a complex cascade of events and the pathogenesis of AP remains still obscure and multifactorial
Thirty male specific pathogen-free (SPF) Sprague Dawley (SD) rats weighing 180∼ 200 g were obtained from the Animal Biosafety Level III laboratory (ABSL-III lab) of Wuhan University in China
Our results demonstrated that Shen-Fu injection (SFI) inhibited the production of proinflammatory cytokines, the expression of NF-κB, and serum levels of lipase and amylase in caerulein-induced rat acute pancreatitis
Summary
Acute pancreatitis (AP) involves a complex cascade of events and the pathogenesis of AP remains still obscure and multifactorial. The proinflammatory cytokines (such as IL-1β and TNF-α) and the anti-inflammatory cytokines (such as IL-4 and IL-10) have been shown to be intimately involved in the inflammatory response to AP [2]. NF-κB is activated during early stages of pancreatitis and regulates many genes that may control inflammatory activities. There is no specific treatment available for AP, numerous pharmacologic therapies have been evaluated over the past few decades. A few of them such as anti-inflammatory medications and antioxidants have demonstrated promise in significantly altering the progression of AP [4], and the search for specific therapies continues
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
