Abstract
BackgroundIntestinal injury is a potential cause of death after high-dose radiation exposure. The aim of the present study was to investigate the protective effects of resveratrol against radiation-induced small intestine injury.MethodsC57BL/6 N mice were irradiated and treated with resveratrol and/or Ex527 (a potent Sirt1 inhibitor), and subsequent examining intestinal morphological changes, and crypt cell apoptosis. Then, the expression and enzyme activity of SOD2 in the small intestine were examined. Furthermore, Sirt1 and acetylated p53 expression was analysed.ResultsCompared to the vehicle control, treatment with resveratrol improved intestinal morphology, decreased apoptosis of crypt cells, maintained cell regeneration, and ameliorated SOD2 expression and activity. Resveratrol also regulated Sirt1 and acetylated p53 expression perturbed by irradiation in the small intestine. The protective effect of resveratrol against ionizing radiation induced small intestine injury was significantly inhibited by Ex527.ConclusionOur results suggest that resveratrol decreases the effects of radiation on intestinal injury at least partly via activation of Sirt1.
Highlights
Intestinal injury is a potential cause of death after high-dose radiation exposure
Ionizing irradiation (IR)-mediated toxicity is largely defined as clonogenic cell death and apoptosis in the crypt cells, resulting in insufficient replacement of villus epithelium, breakdown of the mucosal barrier leading to mucositis, and prominent inhibition in the compensatory proliferative reactions [2, 3]
Mice treated with Rev. and EX527 were similar to IR group, and the body weight did not increase
Summary
Intestinal injury is a potential cause of death after high-dose radiation exposure. The aim of the present study was to investigate the protective effects of resveratrol against radiation-induced small intestine injury. Results: Compared to the vehicle control, treatment with resveratrol improved intestinal morphology, decreased apoptosis of crypt cells, maintained cell regeneration, and ameliorated SOD2 expression and activity. The protective effect of resveratrol against ionizing radiation induced small intestine injury was significantly inhibited by Ex527. The toxic effects of RT on patients include anorexia, vomiting, diarrhea, dehydration, systemic infection, and in extreme cases, septic shock and death [4, 5]. Such side effects may lead to a reduced quality of life for the patient and necessitate radiation dose reduction, which limits treatment success
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