The protective effects of gangliosides on LPS-induced PC12 cells injury

Abstract

To investigate the protective effects of endogenous gangliosides on LPS-induced PC12 cells injury and its possible mechanism. PC12 cells were cultured, and lipopolysaccharide(LPS) injury model was established. We detected the changes in sur-vival rate of different concentrations of LPS on PC12 cells, and the changes in survival rate of LPS when endogenous gangliosides were exhaust-ed by D-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol(D-PDMP), and the protective effects of monosialoganglioside (GM1) on LPS-induced PC12 cells injury. Meanwhile, we observed the morphological changes of GM1 on PC12 cells induced after LPS injury by inverted microscope and fluorescence microscope, and then we detected the relative expression of NF-κB. LPS could decrease the survival rate of cells in a concentration-dependent manner, and GM1 could significantly protect the cells against the changes in survival rate and morphologi-cal damages induced by LPS; After depletion of endogenous gangliosides by D-PDMP, LPS had more injury to PC12 cells, which could be im-proved by adding GM1; RT-PCR results showed that the relative expression of NF-κB in PC12 cells was increased in LPS group, while relative expression of NF-κB increased much higher when endogenous gangliosides were exhausted by D-PDMP, and the relative expression of NF-κB was decreased after GM1 being added. The endogenous gangliosides might through NF-κB signal pathway to protect PC12 cells a-gainst LPS-induced injury.