Abstract
The purpose of this study was to observe the effects of a novel combination of inositol-stabilized arginine silicate complex (ASI) and magnesium biotinate (MgB) on the prevention of skin damage after UVB exposure in rats. Forty-nine Sprague-Dawley rats were randomized into one of the following groups: (1) NC, normal control, (2) SC, shaved control, (3) UVB (exposed to UVB radiation), (4) ASI+MgB-L (Low Dose), (5) ASI+MgB-H (High Dose), (6) ASI+MgB-L+MgB cream, (7) ASI+MgB-H+MgB cream. The results showed that ASI+MgB treatment alleviated the macroscopic and histopathological damages in the skin of rats caused by UVB exposure. Skin elasticity evaluation showed a similar trend. ASI+MgB increased serum Mg, Fe, Zn, Cu, Si, biotin, and arginine concentrations and skin hydroxyproline and biotinidase levels while decreasing skin elastase activity (p < 0.05) and malondialdehyde (MDA) concentration (p < 0.001). Moreover, ASI+MgB treatment increased skin levels of biotin-dependent carboxylases (ACC1, ACC2, PC, PCC, MCC) and decreased mammalian target of rapamycin (mTOR) pathways and matrix metalloproteinase protein levels by the regulation of the activator protein 1 (AP-1), and mitogen activated protein kinases (MAPKs) signaling pathways. In addition, ASI+MgB caused lower levels of inflammatory factors, including TNF-α, NFκB, IL-6, IL-8, and COX-2 in the skin samples (p < 0.05). The levels of Bax and caspase-3 were increased, while anti-apoptotic protein Bcl-2 was decreased by UVB exposure, which was reversed by ASI+MgB treatment. These results show that treatment with ASI and MgB protects against skin damage by improving skin appearance, elasticity, inflammation, apoptosis, and overall health.
Highlights
Various intrinsic and extrinsic factors influence skin aging
UVB exposure caused a marked decrease in skin hydroxyproline levels and biotinidase activity by 46.1 and 42.8% compared to shaved rats (Table 1; p < 0.001)
Elastase activity in the dorsal skin increased from UVB irradiation by 53.3% compared to shaved rats (p < 0.05), whereas arginine silicate complex (ASI) + magnesium biotinate (MgB) treatments exerted protection against the increase in dorsal skin elastase activity induced by UVB irradiation (p < 0.05)
Summary
Various intrinsic and extrinsic factors influence skin aging. Internal skin aging, termed ‘spontaneous aging,’ is the physiological changing of the skin affected by genetic factors and occurs naturally over time (Farage et al, 2008). Photoaging from chronic UV exposure leads to a complex skin-changing process that occurs predominantly on cutaneous surfaces exposed to the sun (Gragnani et al, 2014). ROS mediates harmful posttranslational properties on aging skin by direct chemical alterations to DNA, cell lipids, and dermal matrix proteins, including collagens (Gragnani et al, 2014). All these processes result in an irregular and non-functional accumulation of elastic fibers in the skin. Chronic exposure to solar radiation causes multiple skin disorders, including sunburn, irregular pigmentation, and skin cancer, non-melanoma skin cancers (Dunaway et al, 2018)
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