Abstract
The present study aimed to explore the protective effect and molecular mechanisms of Trichilia catigua A. Juss. extract (TCE) against di (2-ethylhexyl) phthalate (DEHP)-induced damage to the reproductive system of mice. Acute toxicity tests revealed that the maximum tolerated dose (MTD) in mice was up to 2.7 g kg−1. After induction with DEHP, TCE (L-TCE, M-TCE, H-TCE) was orally administered to mice for 28 days. Differences in indicators among groups showed that TCE significantly improved the anogenital distance and the organ indexes of the epididymides and testes. It also significantly reduced varicocele and interstitial cell lesions compared to the model group. H-TCE reduced the sperm abnormality rate, increased the levels of sex hormones, Na+K+ and Mg2+, Ca2+-ATPase enzyme activity, antioxidant enzyme vitality, coupled with a significant decrease in LH and MDA contents. The levels of testicular marker enzymes ACP and LDH were significantly augmented by both M-TCE and H-TCE. Further studies claimed that DEHP induction reduced the mRNA expression levels of Nrf2, SOD2, SOD3, CDC25C CDK1, CYP11A1, 3β-HSD, 5ɑ-R, AR, SF1, and CYP17A1, increased the level of Keap1, while TCE reversed the expression levels of these genes. Meanwhile, IHC results demonstrated a significant change in the expression activity of the relevant proteins compared to the control group. The results suggest that M-TCE and H-TCE enabled the recovery of DEHP-induced reproductive system damage in male mice by improving testicular histopathology, repairing testicular function, and reducing oxidative stress damage. The oxidation-related Keap1-Nrf2 pathway, SODs enzyme, the cell cycle control-related CDC25C-CDK1 pathway, and the steroidogenic-related pathway may contribute to this protective effects of TCE.
Highlights
Several predisposing factors that are responsible for the decline in sperm quality and count have been reported in the past 50 years (Damstra et al, 2002; Merzenich et al, 2010)
The results suggest that M-Trichilia catigua A. Juss. extract (TCE) and H-TCE enabled the recovery of di (2-ethylhexyl) phthalate (DEHP)-induced reproductive system damage in male mice by improving testicular histopathology, repairing testicular function, and reducing oxidative stress damage
Limited improvement in testicular pathology with relief of varicocele dilation, atrophy, and interstitial cell hyperplasia was recorded in the L-TCE, M-TCE, and H-TCE groups, with the best improvement reported by the M-TCE group
Summary
Several predisposing factors that are responsible for the decline in sperm quality and count have been reported in the past 50 years (Damstra et al, 2002; Merzenich et al, 2010). Studies have reported that DEHP interferes with the reproductive endocrine system, reducing sperm motility, decreasing sperm count, causing atrophy and morphological abnormalities in parenchymal organs such as the testis, and is highly toxic to the male reproductive system (Saunders et al, 1997; Lyche et al, 2009; Abd-Ellah et al, 2016). The pathophysiological mechanism behind phthalate-induced atrophy in several tissues may be attributed to oxidative stress (Farombi et al, 2007; Muczynski et al, 2012). These are involved in deregulation in the balance of antioxidant defense in tissues and induction of peroxidative damage (Andrade et al, 2006). Several studies have documented that oxidative damage induced by phthalates can lead to DNA damage and impaired mitochondrial function, reducing the activity of genes associated with high pass synthesis, impeding organ development and testicular sperm production (Yeung et al, 2011; Barakat et al, 2017; Barakat et al, 2019)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.