THE PROTECTIVE EFFECT OF SESAME OIL AGAINST RENAL TOXICITY INDUCED BY CCL4 IN EXPERIMENTAL MODEL

Abstract

Objective: Renal toxicity is one of the most common kidney problems that occur when the body is subjected to drugs or chemical reagents as carbon tetrachloride (CCl4). The aim of present study was to investigate the protective effect of a daily oral dose of sesame oil on oxidative stress, lipid peroxidation and DNA damage associated renal injury induced by CCl4 injection. Renal injury was induced in rats by interaperitoneal injection of 10% CCl4 dissolved in olive oil twice a week in a dose of 1ml/kg, another group of rats simultaneously received sesame oil orally at a dose of 1ml/kg for six weeks. At the end of the experimental period, the protective effect of sesame oil was measured on kidney injury molecule (KIM-1), oxidative stress (malondialdehyde (MDA)), total antioxidant capacity (TAC) and DNA damage. The present study found that sesame oil inhibited CCl4-induced renal injury, lipid peroxidation, DNA damage and increased antioxidant capacity. It was found that co-administration of sesame oil along with CCl4 ameliorated the toxic effect of CCl4 which led to a significant decrease of urea, creatinine, MDA and significant increase in glutathione (GSH) levels, also decrease DNA fragmentation by reduced olive tail moment and the % of tail DNA and decrease KIM1 expression. Co-administration of sesame oil along with CCl4 increased antioxidant capacity by increasing nuclear factor-erythroid-2-related factor (Nrf2) and TAC levels. We hypothesize that a daily sesame oil supplement attenuates oxidative stress associated renal injury by reducing oxygen free radicals and lipid peroxidation in CCl4 treated rats and increased antioxidant capacity.