The protective effect of Rosa canina distilled water on ischemia-reperfusion injuries in the isolated rat heart

Abstract

In recent years, postconditioning by natural pharmaceutical agents has been considered as a treatment for injuries due to ischemia/reperfusion (IR). Thus, in the present study, the effect of Rosa canina distilled water on ischemia and reperfusion experimental myocardial injury was investigated. Male Wistar rats were divided into five groups (n=7); one control IR group and four treated groups, with different doses of Rosa canina distilled water, 0.416%, 1.25%, 2.5% and 4.16% were added to perfusate at the final 5 minutes of ischemia and the first 15 minutes of reperfusion. The isolated rat hearts were fixed on the Langendorff system, and after a period of equilibrium were subjected to 30 min topical ischemia and 60 min reperfusion. Heart rate (HR), left ventricular developed pressure (LVDP) and rate-pressure product (RPP) were investigated. Myocardial infarct size was determined at the end of reperfusion. Compared with the IR group, 0.416% and 1.25 % decreased the HR at the end of ischemia significantly, the 1.25% and 2.5% groups significantly improved LVDP and RPP, and all treatment dilutions decreased myocardial infarct size. Rosa canina distilled water showed positive inotropic and negative chronotropic effects and induced cardioprotection in a dose-dependent manner.