Abstract
The aim of this study is to investigate the protective effect of Radish (Raphanus sativus) seed alcoholic extract 70% against oxidative stress induced by sodium nitrite NaNO2 Twenty five adult male rabbits were devided into five groups of (five rabbits in each group) and treated daily for 30 days. Group T1: intubated orally 20 mg/kg NaNO2, Group T2: intubated orally 20 mg/kg NaNO2 + 50 mg/kg of alcoholic extract from Raphanus sativus seeds, Group T3: intubated orally 20 mg/kg NaNO2 + 100 mg/kg of alcoholic extract from Raphanus sativus seeds, Group T4: intubated orally 20 mg/kg NaNO2 + 200 mg/kg of alcoholic extract from Raphanus sativus seed as well as Group C: control intubated orally distilled water. In comparison with normal rabbits there were significantly increase in the level of Methemoglobin (MetHb%) , lipid peroxidation indicator malondialdehyde (MDA), and significantly decrease in the level of glutathione (GSH) in the rabbits intubated NaNO2. In rabbits intubated NaNO2 + 50,100,200 mg/kg of alcoholic extract it was found there were significant decreased in the level of MetHb%, MDA, and increase in the level of GSH. In conclusion according to results obtained from this study approved that alcoholic extract of Raphanus sativus seeds in dose (50,100,200 mg/kg) have protective effect against oxidative stress induced by sodium nitrite in male rabbits.
Highlights
Food Additives are used for maintaining the high quality of foods
An inorganic salt is used as a preservative and a color fixative in meats and fish it stabilizes the color of preserved fish and meats and it inhibits the growth of Clostridium botulinum[1]
The results of the present study pointed to significant change in MetHb level in group treated with sodium nitrite
Summary
Food Additives are used for maintaining the high quality of foods. Food preservatives are the additives that are used to inhibit the growth of bacteria, molds and yeasts in the food. Free radicals are reactive molecules these have one or more unpaired electrons and are naturally produced in human body by natural biological processes or may be resulting from an outside source (such as tobacco smoke, toxins, or pollutants) These free radicals are toxic compounds that can attack and damage biomolecules, including proteins, nuclei acids and lipids. Previous studies suggest that intracellular reactive oxygen species (ROS) production is a possible mechanism underlying nitrite toxicity These reactive oxygen species (ROS) induce lipid peroxidation (LPO), a chain process which affects unsaturated fatty acids mainly localized in cell membranes leading to generation of malondialdehyde. These damages can be neutralized with antioxidant systems such as glutathione, glutathione reductase, etc.
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