The protective effect of hypoxic-preconditioned BMSCs on spinal cord tissue after ischemia reperfusion injury in rats

Abstract

Objective To investigate the protective effect of hypoxic-preconditioned bone marrow mesenchymal stem cells (BMSCs) on spinal cord tissue after ischemia reperfusion injury. Methods Healthy adult Sprague Dawley (SD) rats weighing 200~250 grams (g) were randomly divided into 3 groups with 6 animals in each group: The sham group received simple surgical manipulation without ischemia/reperfusion treatment; The spinal cord ischemia/reperfusion group (Control group) only received spinal cord ischemia/reperfusion surgery. The hypoxic preconditioned BMSC transplantation group (HP-MSCs group) was injected with hypoxic preconditioned BMSCs 2 days before ischemia/reperfusion. The control group, HP-MSCs group received spinal cord ischemia/reperfusion for 10 min and observed for 48 h. The permeability of the blood-spinal cord barrier was examined with Evans blue (EB), and the histomorphology changes were observed with hematoxylin and eosin (HE) staining. Results EB red fluorescence was significantly weakened in the HP-MSCs group than that in the Control group (P<0.05=, and more intact motor neurons were found in the lumbar spinal cords in the HP-MSCs group than that in the Control group(P<0.05=. Conclusions The hypoxic-preconditioned BMSCs could effectively attenuate spinal cord ischemia reperfusion injury, it may be associated with protective effect of the blood-spinal cord barrier integrity. Key words: Anoxia; Hematopoietic stem cell transplantation; Mesenchymal stem cell transplantation; Spinal cord ischemia/SU; Reperfusion injury/SU