The protective effect of collagen peptides from bigeye tuna (Thunnus obesus) skin and bone to attenuate UVB-induced photoaging via MAPK and TGF-β signaling pathways

Abstract

The aim of this study was to investigate the protective effect and mechanism of bigeye tuna (Thunnus obesus) skin collagen peptides (TSCP) and bone collagen peptides (TBCP) on skin photoaging. In UVB-damaged NIH/3T3 cells, TSCP and TBCP could effectively scavenge excessive reactive oxygen species, suppress mRNA and protein expression of MMP-1, and inhibit the decreased mRNA and protein expression of TGF-β1 and type I collagen. In the ICR mice model exposed to UVB, TSCP and TBCP could effectively improve moisture content, Hyp content and total antioxidant capacity level, activate TGF-β1 signaling pathway, and inhibit MAPK signaling pathway, thereby retarding UVB-induced skin photoaging. TSCP exhibited more excellent antioxidant activity, inhibited p-p38 activation and promoted TGF-β1 expression via TGF-β signaling pathway. By contrast, TBCP was more potent to down-regulate protein expression of p-ERK, p-JNK and MMP-1. The present work provides a theoretical basis for employment of collagen peptides to attenuate UVB-induced photoaging.