The Protective Effect of Carotenoids, Polyphenols, and Estradiol on Dermal Fibroblasts under Oxidative Stress.

Aya Darawsha,Yoav Sharoni,Joseph Levy,Aviram Trachtenberg

doi:10.3390/antiox10122023

Aya Darawsha, Yoav Sharoni + Show 2 more

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https://doi.org/10.3390/antiox10122023

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Abstract

Skin ageing is influenced by several factors including environmental exposure and hormonal changes. Reactive oxygen species (ROS), which mediate many of the effects of these factors, induce inflammatory processes in the skin and increase the production of matrix metalloproteinases (MMPs) in dermal fibroblasts, which leads to collagen degradation. Several studies have shown the protective role of estrogens and a diet rich in fruits and vegetables on skin physiology. Previous studies have shown that dietary carotenoids and polyphenols activate the cell’s antioxidant defense system by increasing antioxidant response element/Nrf2 (ARE/Nrf2) transcriptional activity and reducing the inflammatory response. The aim of the current study was to examine the protective effect of such dietary-derived compounds and estradiol on dermal fibroblasts under oxidative stress induced by H2O2. Human dermal fibroblasts were used to study the effect of H2O2 on cell number and apoptosis, MMP-1, and pro-collagen secretion as markers of skin damage. Treatment of cells with H2O2 led to cell death, increased secretion of MMP-1, and decreased pro-collagen secretion. Pre-treatment with tomato and rosemary extracts, and with estradiol, reversed the effects of the oxidative stress. This was associated with a reduction in intracellular ROS levels, probably through the measured increased activity of ARE/Nrf2. Conclusions: This study indicates that carotenoids, polyphenols, and estradiol protect dermal fibroblasts from oxidative stress-induced damage through a reduction in ROS levels.

Highlights

The skin is affected by environmental factors such as exposure to UV radiation, air pollutants, xenobiotics, and cigarette smoke [1,2], and by endogenous changes that occur with ageing [3]
Results areare presented as tion of matrix metalloproteinases (MMPs)-1 (b) and pro-collagen were determined as described in the legend
The protection of dermal by carotenoids, rotenoids, polyphenols, andshown estradiol shown in the current study may to beskin relevant to skin polyphenols, and estradiol in the current study may be relevant damage that damage that is caused by several factors such as radiation and environmental polluis caused by several factors such as UV radiation and environmental pollutants, inducing tants, inducing the generation of reactive oxygen species (ROS)

Summary

Introduction

The skin is affected by environmental factors such as exposure to UV radiation, air pollutants, xenobiotics, and cigarette smoke [1,2], and by endogenous changes that occur with ageing [3] These factors generally increase oxidative stress by generating reactive oxygen species (ROS) [1,2]. Oxidative stress can cause skin damage due to induction of apoptotic cell death [9,10]; activation of inflammatory processes [1]; an increase in metalloproteinases (MMPs), which degrade collagen in the extracellular matrix [11,12]; and a reduction in collagen production [13] All of these changes accelerate skin ageing.

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